Tag: gene synthesis

Gene Synthesis Plasmid Preparation

At Synbio Technologies we pride ourselves in being one of the premier companies within not only the gene synthesis industry, but the biotechnology industry as a whole. This confidence is relying upon our tested and proven methods of gene synthesis. Gene synthesis can be defined as the method in synthetic biology used to engineer an artificial gene of interest in a laboratory setting. Ever since gene synthesis was first successfully conducted in the early 1970s it has become a highly sought mechanism in various fields of genetics research. For this reason many companies, like Synbio Technologies, have been attempting to optimize their gene synthesis products; but none of them have done this with quite success like Synbio Technologies. Our Syno Platform allows us to generate any sequence of interest up to and including 200kb in length. Another aspect of gene synthesis that Synbio Technologies has to offer is our gene synthesis plasmid preparation. Using this technology we are capable of generating any sequence and inserting it into any plasmid of interest. For gene synthesis plasmid preparation there are typically two forms of final product: transfection grade and research grade. Both differ slightly in their mechanism of being generated, as well as research application. Through use of Synbio Technology’s Syno®Platform, as well as our gene synthesis plasmid preparation we are ready to supply our customers with whichever plasmid product is necessary.

The differences between the transfection grade and research grade generated plasmids lies mainly in the applications each particular plasmid is used for. First, the research grade is used more in a laboratory setting to conduct various types of genetics research. These topics include: molecular cloning, mutagenesis, southern blotting, etc. All of these methods are used in order to conduct various types of genetics research with use of gene synthesis. Where gene synthesis comes in is through the insertion of the requested sequence into the plasmid. This insertion, and subsequent amplification, allows our customers to conduct any type of research necessary that requires a research grade plasmid construction. Second, the transfection grade plasmid is used mainly for protein manufacturing, antibody production and other forms of gene therapy. The link between this plasmid and gene synthesis is again the insertion of the sequence of interest into the particular plasmid. This can be extremely useful when conducting different types of gene therapy. The requested sequence can be loaded into the plasmid with the hopes of reversing the endogenous mutated gene of interest. This powerful technology has the ability to further our understanding of genetics and it is all thanks to gene synthesis.

Gene synthesis is something that Synbio Technologies does extremely well, mainly relying upon our Syno®Gene Synthesis Platform. This platform is used to generate the customer requested sequence with one hundred percent accuracy. The resulting gene synthesis product can then be used in order to prepare a plasmid of interest. This process is normally quite daunting, but Synbio Technologies is more than confident in our ability to generate the requested gene synthesis product and resulting gene synthesis plasmid. This process is all done within a quick turnaround time and competitive prices. For this reason, Synbio Technologies has risen to the top of not only the gene synthesis industry, but the resulting biotechnology industry as well. We offer a one stop shop for our customer’s gene synthesis plasmid preparation, with competitive prices, high quality output, and an efficient turnaround time. With this combination, our customers will be conducting research in no time with confidence in the product that we supply them.

Gene Synthesis Related Services

Synbio Tenchologies can also design sequencing with codon optimization software -NGTMCodon Optimization Technology at no cost.

Gene Synthesis Definition

The traditional method of studying a gene’s function is to extract the gene of interest, amplify it and put the resulting sequence through various molecular assessments. These assessments can vary from generating a frameshift mutation within the sequence, to upregulating the expression of the sequence in order to observe the resulting phenotypes the mutated sequence has induced on the organism. All of these methods are particularly important within the field of genetics research, but the methods of extracting these sequences to then mutate and study is becoming laborious and cumbersome. One, relatively recent and groundbreaking method, gene synthesis , has taken this traditional method of studying genetics and introduced some interest alterations. Gene synthesis is defined as the synthetic biology method used to engineer artificial genes within a laboratory setting. This method has allowed us, at Synbio Technologies, to go from a sequence in text format to physical copy of the genetic sequence itself with ease and extreme accuracy. Ever since gene synthesis was successfully conducted in 1972, by Hard Gobind Khorana and associates, this technology has been growing not only in popularity but in effectiveness and efficiency. One of the main reasons this technology has increased in popularity is that it allows for the creation of long, up to 200kb, sequences to be synthesized with extremely high accuracy. With the capability of generating sequences this large, gene synthesis gives a powerful approach to studying various topics such as complex pathways and generating synthetic DNA libraries. This high accuracy and relatively long length have promoted gene synthesis to be applied to various fields of genetics and biological research topics.

The fields of biological research that gene synthesis has been applied to vary from targeted mutagenesis in order to study a gene’s function to agriculture through increasing crop production. One of the main advantages that gene synthesis offers it the ability for a customer to request a specific sequence of DNA in text format up to 200kb in length. This text format is then converted into a physical copy with relative ease and high accuracy. The resulting sequence is then amplified an adequate amount of times in order to comply with the customer’s request. The accuracy allows the customer to generate a mutation anywhere within a gene of interest specific to their research topics. Not only can the mutation be generated anywhere, but any type of mutation can be used: frameshift, deamination, deletion, insertion, or nonsense mutation. In addition to this, gene synthesis can also be used to upregulate a gene of interest in order to cause an overexpression of the resulting phenotype. All of these mutations can be requested and inserted or applied the sequence of interest in order to obtain a better understanding of different types of genetics research such as evolution, or antibiotic resistance. In addition to these applications, gene synthesis has also been applied to agriculture through use of genetic engineering. Gene synthesis is the basis of genetic engineering, allowing for the creation of a resistance gene to a particular bacteria for example. The advantage that gene synthesis has to offer here is the ability to generate mutations within the synthesized gene to keep up with the constantly evolving bacteria. Gene synthesis through genetic engineering, have helped grow the agriculture industry, as well as develop a better understanding on how to improve crop survivability in varying climates as well as exposure to certain harmful bacteria. These applications have large impacts on not only the field of genetics, but the world. This is the main reason as to why Synbio Technologies is proud to be one of the premier companies within the gene synthesis industry.

At Synbio Technologies, we try to keep our gene synthesis process as simple as its definition. Gene synthesis is carried out with ease and extremely high accuracy through our Syno®Gene Synthesis Platform. This three phase platform allows us to generate the physical genetic sequence of the customer specified request. This generated sequences is guaranteed to be one hundred percent identical to the customer requested sequence. The guarantee is backed by Sanger sequencing at multiple phases of the three platforms. The resulting gene synthesis product will then be shipped to the customer specific location within an efficient timeframe. This entire process allows Synbio Technologies to offer the highest quality product, with an efficient turnaround time. These two aspects, combined with competitive prices and respectable reputation, it is clear that Synbio Technologies is one of the premier companies within the gene synthesis industry.

Gene Synthesis Related Services

How to order

Get a quote: online request submission form.

Submit gene/protein sequence: Gene synthesis online inquiry.

Or please email your detailed sequences and requirements directly to: service@synbio-tech.com, Tel.: +1 732-230-3003. Project managers of Synbio Tech. will contact you within 24 hours after we receive your request.

Large Gene Synthesis

An interesting and important aspect of genetic research that has grown drastically in popularity over the last ten to fifteen years is gene synthesis . Gene synthesis can be defined as the construction of a physical genetic sequence from a requested sequence in text format. Gene synthesis allows us, at Synbio Technologies, to manufacture genetic sequences that may not already exist within nature. In addition to the de novo sequences generated, sequences can also vary greatly in length, from short sequences to large sequences up to 200kb. This process is carried out with Synbio Technology’s, patent pending, Syno®Platform. This three stage platform makes going from requested costumer specific sequence to physical copy of the sequence with ease. One aspect in particular that the Syno®Platform is known for is large gene synthesis. The Syno®platform has the ability to synthesize large genes up to and including 200kb in length. The wide range of possibilities makes Synbio Technology’s Syno®Platform attractive and sought out by researchers all over the world especially when it refers to large gene synthesis. This has led Synbio Technologies to become one of the premier companies within the realm of not only large gene synthesis, but any length gene synthesis. This confidence in our large gene synthesis relies upon one of the two most popular methods of gene synthesis, proven time and time again.

There are two common methods of large gene synthesis: Gibson Assembly Method and Yeast homologous recombination technology. The Gibson Assembly Method now the most common method of achieving directed cloning and in vitro multiple-segment assembly. The Gibson Assembly Method is commonly used by various companies to conduct gene synthesis for varying lengths. It is an effective method for gene synthesis, but the one major restriction that comes along with this method is the inability to easily synthesize fragments over 20kb in length. This is an acceptable method for small gene synthesis, but a different method is needed for large gene synthesis. The yeast homologous recombination technology has the ability to assemble fragments up to 1.08Mb in length. This length is larger than the average prokaryotic genome, making yeast homologous recombination technology a reliable method to use for both large and small gene synthesis. It is yeast homologous recombination that is utilized for Synbio Technology’s Syno®2.0 and Syno®3.0 platforms, the foundation of gene synthesis at Synbio Technologies. It is this technology that allows us to provide one-stop services which includes: basic gene synthesis, gene cluster, and large gene synthesis. With this technology, the Syno®Platforms not only makes it possible to synthesize large genes, but small genomes as well. In addition to the large gene synthesis, Synbio Technology also offers the ability to guarantee 100% sequence verification. This is carried out using Sanger sequencing at multiple steps of the Syno®Platform stages in order to verify the sequence accuracy. The sequencing verification is necessary when dealing with large gene synthesis. The accuracy of sequences of this length is not always guaranteed to be one hundred percent to the identical sequence with other companies, but with our Syno®Platform it is.

At Synbio Technologies we are more than confident in our abilities to generate your requested genetic sequence, up to and including 200kb in length. The possibility of synthesizing a gene this large has opened up a vast amount of new opportunities and approaches to various topics within genetics research. Large gene synthesis is not a task that is easily accomplished, but at Synbio Technologies we are capable of generating the requested sequence with one hundred percent accuracy in an efficient timeframe. This confidence is relying upon the efficiency and accuracy of our Syno®Platform and how it has been proved its ability to generate genetic sequences up to 200kbs. In addition to this, our prices are at an all-time low, starting at $0.19 per base pair through our gene synthesis promotions. With this confidence in our large gene synthesis protocol and low prices we are more than ready to offer you the highest quality product for an extremely competitive price.

Gene Synthesis Related Services

Synbio Tenchologies can also design sequencing with codon optimization software -NGTMCodon Optimization Technology at no cost.

Gene Synthesis Vectors

A relatively recent discovery, dating back to the early 1970s, in biology has revolutionized the field of genetics research. This discovery is known as gene synthesis , which allows for the manufacturing of a physical genetic sequence from a string of base pairs in text format. Gene synthesis allows for the creation of a genetic sequence that may not be preexisting within nature. Previously, a sequence of interest was required to be present within an organism in order to be extracted and later amplified to study its function. Gene synthesis allows us, at Synbio Technologies, to bypass this requirement entirely. This is accomplished through our, patent pending, Syno®Platform, which allows us to move from a sequence in text format to a physical copy with ease and accuracy. One common advantage that gene synthesis offers is the construction of gene synthesis vectors. Gene synthesis vectors vary widely in structure, function, length, and physical sequence characteristics. With the use of Synbio Technology’s Syno®Platform we are capable of not only synthesizing any gene of interest, up to 200kb in length, but constructing any gene synthesis vector specified by the customer. The generated gene synthesis vector can then be used in a wide range of applications, specific to each type of gene synthesis vector.

There are three main types of gene synthesis vectors that Synbio Technologies prides itself in its ability to generate with high accuracy in order to better suite the customer’s particular request. The three vectors are: short hairpin RNA (shRNA), transcription activator-line effector nuclease (TALEN), and CRISPR-Cas9. All three have similar functions, gene knockout also known as gene silencing, but have various methods in order to accomplish this. shRNA use RNA interference in order to block the transcription and translation of the gene/sequence of interest, rending it inactive. TALEN and CRISP-Cas9 use genome editing in order to render the gene/sequence of interest inactive. All three have been tested and proven as effective methods of gene silencing and inhibiting the resulting protein expression. Gene silencing is a common method within genetics research in order to determine the function of a gene. Once the gene is rendered inactive, researchers can study the resulting phenotype and infer on the function of gene of interest. Where gene synthesis comes into play is through our Syno®2.0 and Syno®3.0 Platforms Synbio Technologies is capable of constructing the requested sequence within any of these vectors, in addition to other popular vectors. This technology allows the customer to design a sequence, in text format, which allows Synbio Technologies to generate the requested gene synthesis vector. Once generated, the sequence and resulting vector will work to inhibit the endogenous gene, lowering protein expression and essentially silencing the gene of interest. As mentioned before, this silencing is achieved through the various methods of gene silencing utilized by each vector.

At Synbio Technologies we are extremely confident in our ability to construct these gene synthesis vectors to the customer’s exact specifications. We guarantee with one hundred percent accuracy within our generated sequences in order to produce the highest quality output. With this accuracy we can guarantee the best possible method of achieving a gene knockout through the use of our gene synthesis vectors. At Synbio Technologies we don’t only offer the highest quality product, but extremely fast turnaround times and competitive prices as well. Within as few as 10 days the customer’s gene synthesis vector will be constructed and be shipped to the customer specified location. With this confidence, high quality output, fast turnaround time, and competitive prices it is clear the Synbio Technologies is one of the leading companies within the gene synthesis industry.

Gene Synthesis Related Services

Synbio Tenchologies can also design sequencing with codon optimization software -NGTMCodon Optimization Technology at no cost.

Gene Synthesis Promotion

After its discovery and first successful application of gene synthesis in the early 1970s, by Har Gobind Khorana and associates, it has become a frequently used method within genetics and other biological research fields. Gene synthesis has a simple definition but long lasting impacts on the genetics research community and various other fields of research. Gene synthesis is the creation of a physical DNA sequence in a laboratory setting through use of various technologies. The process can easily take a requested sequence in text format and generate the physical copy of the requested sequence with ease and accuracy. Gene synthesis offers the unique ability to generate and later amplify genetic sequences that do not exist naturally in nature. This has many advantages in various fields of genetics research such as anti-biotic resistance and other evolutionary studies. At Synbio Technologies this process is accomplished through our, patent pending, Syno Platform. This platform contains three distinct phases that make going from text format to physical copy with ease and efficiency. In addition to this, Synbio Technologies offers various gene synthesis promotions that keep our prices competitive within the gene synthesis industry. The main gene synthesis promotion that we are currently offering is gene synthesis at only $0.19 per base pair. Normally $0.25 a base pair, this gene synthesis promotion is not one that can be easily passed up on. We keep our prices competitive, through our various gene synthesis promotions, and our quality guaranteed. With this combination it is possible to apply gene synthesis to various fields of genetics research with ease and for a competitive price.

Gene synthesis has applications in many different aspects of genetics research. It is applied throughout the medicinal community when studying cancer genetics and developing more effective vaccinations. Gene synthesis is also seen in the agricultural community when developing genetically modified crops that can better withstand any conditions, from arid summers to freezing winters and everything in between. Synbio Technologies is ready to offer an easier and cost effective approach to achieving these various types of research. At Synbio Technologies we offer an experience that is not commonly offered quite anywhere else when it comes to gene synthesis. On average our professional team has over ten years of hands-on experience in gene synthesis, while we average over 10 million base pairs synthesized per month. With this previous experience and constant business we are able to supply our customers with the highest quality output available for the lowest prices through our various gene synthesis promotions. In addition to our experience, using our Syno Platform we have multiple quality assessments in place to ensure the quality of the generated sequence. These quality assessments are used to verify that the sequence being generated by Synbio Technologies is identical to the sequence requested by the customer. At Synbio Technologies we guarantee the accuracy of the sequence, no matter what requested length up to and including 200kbs. As you can see, the gene synthesis promotions that Synbio Technologies has to offer are not limited to the price of synthesizing per base pair. The additional gene synthesis promotions come in the form of our hands-on experience, proven process through our Syno Platform, and high quality output. The combination of these gene synthesis promotions Synbio Technologies offers it is clear that we pride ourselves as being one of the leaders in the gene synthesis industry. Our methods have been tested, proved and verified, and we are confident in our ability to deliver the highest quality product to our customers in an efficient timeframe and cost effective manner. With these advantages you will have your high quality product, for as low as $0.19 per base pair, in a short timeframe and will be better suited to pursue any type of research you and your team are interested in.

Gene Synthesis Related Services

How to order

Get a quote: online request submission form.

Submit gene/protein sequence: Gene synthesis online inquiry.

Or please email your detailed sequences and requirements directly to: service@synbio-tech.com, Tel.: +1 732-230-3003. Project managers of Synbio Tech. will contact you within 24 hours after we receive your request.

Gene Synthesis Mutagenesis

One of the fundamental ways to study the function of a gene is to void, knockout, its function and observe the resulting phenotype. This void of function, commonly known as mutagenesis, has been practice within the field of genetics for many years and has been proven effective countless times. A more commonly utilized method of mutagenesis, site-directed mutagenesis, is one of the most traditional methods of generating a knockout gene. The process of gene synthesis mutagenesis begins with the selection of a short primer. The primer must meet two important characteristics: it must be complementary to the sequence of interest, and it must contain a debilitating mutation that will leave the gene of interest incapacitated. The complementary nature of the primer is necessary for the primer to hybridize with the sequence of interest. The debilitating mutation is necessary in order to generate a knockout in the gene of interest. The single stranded primer is then replicated by DNA polymerase, allowing the amplification of the gene of interest which now includes the mutation specific to the primer. The mutated sequence is then inserted into a bacteria host cell via a viral vector and cloned. After amplification, the resulting sequence is then verified for accuracy. This process is laborious and may also not yield a high quality of resulting sequences. This inefficiency is due to the reliance of multiple different biological methods that are not easy to control. Over the past thirty years there have been multiple additional methods to generate mutated sequences but few with high efficiency. A new method, gene synthesis mutagenesis, has the recently become more popular as a result of its effective results and efficiency. Gene synthesis mutagenesis offers a unique approach to generating a genetic knockout within an organism. Instead of the normal site-directed mutagenesis, and somewhat hoping that the induced mutations will render the gene inactive, gene synthesis mutagenesis has the ability to generate any type of mutation within a gene. This gene synthesis is carried out by our Syno®Platform, which guarantees one hundred percent accuracy of the generated sequence. This guaranteed sequence will now contain a debilitating mutation, resulting in the creation of a knockout with little effect and high probability of success.

Gene synthesis itself has revolutionized the field of genetics. This unique technology offers the ability to synthetically engineer a gene of interest in order to be studied. Since this is possible, it is also possible to generate a gene of interest and insert mutations. This is the basic background of gene synthesis mutagenesis. Gene synthesis is the process of requested genetic sequenced in text format to the engineering of a physical copy of the requested sequence. This process has multiple steps of verification of the sequence of interest. These verifications and overall process can be altered in order to generate a sequence of interest that renders the previously functional gene inactive. Multiple different types mutations can be synthetically engineered, ranging from a frameshift mutation to a large deletion of an entire codon, both of which result in a gene damaged beyond repair. This gene can then be amplified, be either use of polymerase chain reaction or traditional subcloning, and then studied for the effects the knocked out gene has on the organism. The process of gene synthesis mutagenesis completely bypasses the reliance of DNA polymerase replicating the primer necessary in site directed mutagenesis. It relies solely on the ability to generate a mutation in text format, which can easily be done.

The connection between Synbio Technologies and gene synthesis mutagenesis is a simple one, we pride ourselves as being one the leading companies in the gene synthesis industry. On average we synthesize over 10 million base pairs a month. This process is carried out by individuals who on average have been working in this field for 10 years or longer. With this experience and constant business we have been able to perfect the pipeline from original text format to engineered product through our Syno Platform. This format is then slightly altered to accomplish gene synthesis mutagenesis requested by the customer. Instead of using the Syno Platform to synthetically engineer a functioning gene of interest, we engineer a gene of interest with a debilitating mutation. Since Synbio Technologies is one the premier companies in the gene synthesis industry, we can also be considered to be one of the leading companies in the gene synthesis mutagenesis industry.

Gene Synthesis Related Services

Synbio Tenchologies can also design sequencing with codon optimization software -NGTMCodon Optimization Technology at no cost.

Gene Synthesis Cost

Moore’s Law has been applied and shown in various industries ranging from the tech industry to the biotech industry. Moore’s Law has most notably shown within the field of genetics when analyzing the cost of sequencing the human genome. The first whole genome sequencing was conducted on a human in the early 2000s, and costed approximately $100 million dollars. Over the past ten to fifteen years the technology used to sequence the human genome has expanded rapidly, allowing the cost of sequencing to drop significantly as well. The current cost of sequencing a whole human genome is approximately $1,000 dollars, less than 1% of the original cost ten to fifteen years ago. This rapid decline of cost clearly demonstrated a variation of Moore’s Law. Another aspect of genetics that follows Moore’s Law is gene synthesis cost per base pair. Gene synthesis is the engineering of a physical sequence of DNA in a laboratory setting. It has been applied and used in various fields of research with much success. Gene synthesis has had long lasting impacts on the field of genetics due to its unique ability to generate physical copies of genes with ease, efficiency, and accuracy. Similar to the drastic decline in the cost of sequencing a human genome, gene synthesis cost per base pair has dropped from approximately $10 a base pair to approximately $0.10 a base pair over the past 10 years. Similar to the decrease in cost for whole genome synthesis, gene synthesis cost has been driven down by an increase in technology and a large number of companies supplying this technology. At Synbio Technologies we pride ourselves as being one of the leading companies for gene synthesis with some of the lowest prices in the business with the highest quality output.

Although there has been a decrease in gene synthesis cost, there has been an opposite effect on the quality of the output. As cost has fallen, quality and potential gene lengths have risen. At Synbio Technologies we offer the ability to synthesize genes up to and including 200kb in length with one hundred percent accuracy. This is accomplished by our Syno Platform which offers sequence verification multiple times throughout each of the three phases. This process is carried out by use of Sanger sequencing to verify that the generated sequence is identical to the sequence requested by the customer. In addition to the quality, the applications of gene synthesis have increased drastically, causing it to increase in popularity. Gene synthesis is a popular method of research to develop more effective vaccinations as well as genetically modified organisms. All three of these aspects: decreased gene synthesis cost, increased applications, and quality are intertwined. Gene synthesis is a technology that is extremely effective and commonly used in many pipelines by many different researchers. It is a reliable, well tested and trusted technology that has changed genetics research for the better. Pair these advantages with the low gene synthesis cost and it is clear why this method has drastically risen in popularity throughout the genetics community.

The decrease in gene synthesis costs has also caused us at Synbio Technologies to offer some of the lowest prices per base pair with the highest quality output. At Synbio Technologies, we are confident and ready to provide the costumer with the highest quality sequence for the lowest price. The confidence is relying upon our, patent pending, Syno Platform which guarantees one hundred percent accuracy within the synthesized genetic sequenced. This is accomplished with the constant verification of sequence quality for accuracy. This platform in place we can effectively engineering your gene of interest with low cost to you. With the low gene synthesis cost you and your team will be better suited to conduct various types of genetic research specific to your interests.

Gene Synthesis Related Services

Synbio Tenchologies can also design sequencing with codon optimization software -NGTMCodon Optimization Technology at no cost.

Gene Synthesis by PCR

Prior to the 1970s traditional cloning was used in order to amplify a gene of interest. This process of gene synthesis by PCR is still used today, but relies on a laborious process of achieving the final amplified product. Traditional cloning relies on recombinant DNA being integrated into a bacteria host’s genome. Once the DNA is integrated into the bacteria host’s genome, the DNA is then amplified and later extracted. Unfortunately, this process allows for many variables to be interfered with resulting in a tarnished and lower quality product. In the early 1980s, Kary Mullis discovered and developed a much more efficient method of amplifying genes of interest. This method is called polymerase chain reaction (PCR). The basic process that PCR follows starts with the addition of two primers and a heat-stable Taq polymerase to the targeted gene. The mixture is then heated, allowing the hydrogen bonds between strands to denature. The mixture is then cooled, allowing the primers to hybridize to the complementary sequences of target DNA. Heat is then applied again to allow for the Taq polymerase to extend complementary strands from the primers. This process is then repeated hundreds or thousands of times, allowing for a large amount of amplification with ease. PCR has revolutionized the genetics research community, allowing millions of copies of DNA to be synthesized within hours. It is this efficiency that has made PCR become increasingly popular over the past thirty years. Another aspect of genetics that has revolutionized genetics research is gene synthesis. Gene synthesis is the process by which a physical sequence of DNA is constructed from a desired sequence in text format. Gene synthesis is carried out here, at Synbio Technologies, by our Syno®Platform. This allows us, at Synbio Technologies, to synthetically engineer genes up to 100kb in length. The sequence can then be amplified as many times as necessary by PCR. It is this connection between gene synthesis and PCR that has caused a large impact and various applications on the field of genetics.

The connection that these two technologies have is that gene synthesis uses PCR to amplify the gene of interest. The reliance is shown when PCR is used to amplify the synthetically engineered gene to the requested amount of copies. The gene of interest starts in text format, specified by the costumer, and put through our Syno Platform in order to generate the physical copy of the sequence. Throughout this process the possibility for errors within the sequence being generated can be quite high. For this reason, the Syno Platform includes multiple quality assessments to verify that the sequence of interest is identical to the one generated by Synbio Technologies. We offer one hundred percent sequence verification, analyzed with Sanger sequencing. After the sequenced is verified to be one hundred percent accurate, PCR is used to amplify the synthetically engineered gene. The resulting amount of product, specified by the customer, will then be shipped to your location, within as few as five days. The high quality product that the costumer receives from Synbio Technologies can then be put to use in various fields of genetics research that gene synthesis and PCR are commonly associated with. Gene synthesis itself offers a wide range of applications. It has been used to generate more effective vaccinations, as well as to study and improve the delivery of viral vectors used in gene therapy. Gene synthesis is the creation of the sequence of interest, but it is PCR that does the hard work to generate a sufficient amount of the sequence necessary to conduct these types of research. PCR offers the most efficient process to amplify the gene synthesis product in order to obtain a sufficient amount of product. Gene synthesis is a technology that is constantly utilized and relied upon, and few companies offer the efficiency and high quality product that Synbio Technologies offers.

This process, from costumer requested sequence to the final physical product, has been in use by Synbio Technologies for many years and we pride ourselves on our efficiency as well as high quality output. In addition to the efficiency and high quality output, we offer competitive prices and fast turnaround time. For gene synthesis, our prices starts at approximately $0.10 per base pair, which is near the lowest price in the gene synthesis industry. We offer the ability to synthesize a wide range of gene lengths, ranging from 100 base pairs to 100kb base pairs in length with one hundred percent accuracy. In addition to the range of lengths, using our Syno®2.0 Platform, we are capable of generating many different structure of DNA. These structures include: repeated sequences, hairpin structures, high GC percentage, etc. These structures are of course then amplified by PCR in order to fulfill as many copies as the customer may need. After amplification by PCR and before shipping, the engineered sequences are then verified again in order to assure the quality of the product and the conservation of the requested product. This allows us to correct for any errors that may have occurred during the gene synthesis process or amplification by PCR. With the ability to generate large and complicated structures with this high of accuracy it is clear the Synbio Technologies is one of the leading companies in the gene synthesis industry.

Gene Synhtesis Related Services

Synbio Tenchologies can also design sequencing with codon optimization software -NGTMCodon Optimization Technology at no cost.

Molecular Biology Cloning

Molecular biology cloning

Molecular cloning is one method in molecular biology that is commonly used to amplify a genetic sequence of interest. This is accomplished by inserting recombinant DNA into a vector which can then carry DNA fragments in host organisms to be amplified. This process of amplification is based on molecular biology standard, first is to recombine the target gene into the vector DNA molecules in vitro. Then transfer the recombinant DNA to host cells. After transferring, there is a screening of cells which have expressed the recombinant DNA, after purification and amplification.

Molecular Biology Cloning Technology Process:

  • Isolate the target gene and vector:

    • 1.Direct separation is suitable for the extraction and separation of bacterial chromosomes, plasmids and virus DNA whose genetic background are of interest to be studied.
    2.Gene synthesis is used to generate short DNA fragments whose sequence is known clearly.
    3.cDNA can be synthesized by reverse transcription from mRNA.
    4.Screening the gene of from the genomic library for molecular cloning.

  • The target gene and vector are cleaved with a restriction enzyme.

    • This allows the fragments to be more easily connected later.

  • The target gene and vector are then ligated with DNA ligase.

    • This seals the connection between target gene and vector.

  • Transfer the ligated recombinant vector into host cells

    • Bacteria: E. coli, fungi: Yeast, insect cells or mammalian cells.

  • Conduct screening at different levels using different methods to test for quality.

    • For example: vector size, enzyme digestion results, screening markers and so on.

    Molecular biology cloning generally uses DNA sequences from two different organisms. First is the species that is the source of the DNA to be cloned. Second is the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning technology is central to many contemporary areas of modern biology and medicine.

    Molecular Biology Related Services

Introduction to Molecular Biology

Introduction to molecular biology

Molecular biology is a subfield in biology that studies the various topics and molecular mechanisms within cells that are important for proper cellular function. These topics can include any cellular mechanism ranging from cellular metabolism to utilizing a molecular function in order to successfully accomplish gene synthesis. These topics and mechanisms have the ability to be altered slightly to accomplish a particular application of interest. These mechanisms within molecular biology, have been studied intently and applied to various fields of biological research and development. The applications within molecular biology that this article will introduce are gene expression and protein synthesis. These endogenous mechanisms are vital to the proper cellular function within molecular biology that can be altered to successfully conduct both gene synthesis and protein synthesis.

Molecular biology for gene synthesis

One important aspect of the gene synthesis process is that it is not limited to DNA replication of a preexisting DNA fragment. Gene synthesis is capable of in vitro artificial DNA sequence synthesis, which allows for the synthesis of de novo genetic sequences. Contrary to traditional methods of DNA amplification, gene synthesis does not require that the sequence of interest be preexisting within nature. This is accomplished by using refined chemical methods for gene synthesis without a template DNA chain. The resulting templates are then connected to form a gene fragment, by use of various molecular biology mechanisms. With the development of molecular biology, researchers have the ability to synthesize any gene of interest up to and including 200kb in length. The development of more efficient gene synthesis technologies has also allowed the gene synthesis process to become more time efficient, accurate and cost effective. These contributing factors has led gene synthesis to become an important technique within molecular biology.

Molecular biology for protein express

The aim of gene fragment synthesis is for the resulting synthesized gene to be inserted into plasmid vector. After this is accomplished, the recombinant plasmid transcripts are then inserted into cells, where the recombinant plasmid will be expressed. This allows for the expression of the newly synthesized gene of interest and its resulting protein. In order to obtain the highest quality product and strongest protein expression the recombinant protein must first be purified. The resulting purified protein can then be used in subsequent experiments, specific to our customer’s interests. These experiments may include research contributing to the better understanding of protein structure, function and activity of cells, as well as the diagnosis of the certain diseases. All of applications can provide scientific basis for treatment and drug development. For these reasons, optimizing the gene synthesis expression at the protein level is of upmost important to us, at Synbio Technologies.

Synbio Technologies offers comprehensive molecular biology services, designed to fit our customer’s specific needs. These molecular biology services include gene synthesis, PCR cloning, subcloning, site-directed mutagenesis, vector construction and other related projects. The services Synbio Technologies offers rely upon our Syno® 1.0 and Syno® 2.0 platforms. These platforms allow us to generate the highest quality gene synthesis product for a cost effective price. In addition to this, Synbio Technologies also offers the bacterial and yeast expression systems. These systems help optimize the resulting protein product from our synthesized sequences. The whole process is done with such ease and efficiency, that we are confident our high quality purified recombinant protein will arrive to your bench in as soon as 6 weeks.

Molecular Biology Related Services

Long Gene Synthesis

Artificial gene synthesis is a critically important technique in modern synthetic biology. The ability to easily customize and create DNA sequences based off of a single gene, gene clusters, or completely from scratch enables an enormous amount of versatility and a wide range of applications for synthetic DNA.

The necessity of long gene synthesis

Researchers often investigate the genetic causes of a particular target phenotype, frequently by examining the effects of site-directed mutations. Through artificial gene synthesis, inducing mutations, stretches, elongations, or other changes to a target gene sequence becomes substantially easier than before, allowing for efficient study of these mutant phenotypes.

In recent years, the importance of being able to analyze entire genomes or large collections of genes has become more and more pronounced. The necessity of having to look at mutations in the context of an organism’s genome means that being able to synthesize huge amounts of DNA with near-perfect fidelity is becoming increasingly relevant to many current experiments.

Gene Synthesis Scheme

Current solid-phase oligonucleotide synthesis technology can yield chemically synthetic gene constructs. However, it is generally limited to sequences of roughly 200 nucleotides in length. Another approach named Gibson assembly shares a similar drawback in that it is restricted to plasmids of around 10kb or less.

The need for technology that could handle synthesis of very large DNA sequences turned to yeast as the system of choice. Yeast can provide self-connection between multiple DNA fragments easily and rapidly without the application of polymerase or ligase. Synbio Technologies’s own proprietary Syno® platform, combined with the built-in homologous recombination technology of yeast, enables rapid and accurate assembly of various long DNA fragments and genomes of up to 200kb.

Synbio Technologies delivers more than 2 million base pairs of DNA sequences every month, all over the world. Any DNA sequence, even those possessing difficult characteristics such as high or low GC content, hairpin structures, or highly long/complex sequences, is able to be synthesized with 100% accuracy guaranteed. Synbio Technologies can provide large sale and low cost methods to accomplish gene synthesis including assembling long DNA segments at high accuracy and yield.

Gene Synthesis Related Services

How to order

Get a quote: online request submission form.

Submit gene/protein sequence: Gene synthesis online inquiry.

Or please email your detailed sequences and requirements directly to: service@synbio-tech.com, Tel.: +1 732-230-3003. Project managers of Synbio Tech. will contact you within 24 hours after we receive your request.

GC-Rich Gene Synthesis

Gene synthesis is the technology of artificially synthesizing double-stranded DNA in vitro, and is a crucially important technique in molecular biology. Conventional gene synthesis generally involves assembling small segments of DNA and/or amplifying known samples of genetic material, and requires the raw nucleobases guanine, cytosine, adenine, thymine and uracil as starting materials.

GC-content (guanine-cytosine content) refers to the proportion of two nitrogenous bases, guanine and cytosine, in DNA and RNA molecules, which might be any domain of a gene, single gene, gene clusters, or even non-coding regions. Synthesis of high GC-content sequences can be troublesome due to issues with secondary structure, mispriming, or mis-annealing. GC-rich regions tend to facilitate base stacking, which makes them more stable than sequences with low GC-content. Additionally, secondary structures formed by high GC-content regions also tend to be stable and more resilient to denaturation. Sequences containing many guanine repeats can also generate complicated inter-strand folding due to hydrogen bonds between adjacent guanines.

GC-content is closely related to temperature optimization in gene synthesis. In PCR, primer GC ratio strongly influences the predicted annealing temperature of DNA templates. High GC-content tends to require a high melting temperature, which can result in mispriming or mis-annealing between the template and its complementary strand, leading to undesired and inaccurate gene products. Even when GC-rich sequences or G repeats are located in noncoding regions, the secondary strucutres formed can still affect factors in gene synthesis, such as melting temperature. Thus, an important part of gene synthesis is codon optimization, using synonymous codons to lower the GC-content of a sequence and lowering the melting temperature.

Synbio Technologies is proficient at accurately synthesizing error-free DNA constructs meeting customers’ requests, and is capable of assembling multi-kilobase plasmids or even entire genomes. Synbio Technologies can make gene synthesis perfect by handling even the most challenging synthesis requests, including genes or genomes with various complex sequences such as hairpin structures, high GC ratio, high AT percentage, multiple consecutive nucleotide sequences, and so on. The successful synthesis of GC-rich constructs is important to fully understand and study complex genes and even their non-coding segments with high conservation, which in turn poses profound significance in biomedical research.

Gene Synthesis in Genetic Engineering

Gene synthesis has revolutionized what was once thought to be impossible in the field of genetics. Synthesis of the first gene, a yeast tRNA, was achieved by Har Gobind Khorona and coworkers in 1972. Since then this technology has had long last impacts on genetic research and the biotechnology industry. Whether it is synthesizing small genes, ranging in lengths from a few hundred base pairs in length, to large genes, up to 100kb in length, gene synthesis has made recently daunting research aspirations achievable. Gene synthesis has a simple definition, the creation of synthetic genes within a laboratory setting, but an impactful influence on many scientific fields. One field in particular, genetic engineering, is often associated with gene synthesis. The association between these two topics is so prevalent because gene synthesis is a popular mechanism to achieve the genetic engineering sought out by researchers. Genetic engineering also has a wide range of application in many different scientific fields, many relying on gene synthesis.

Gene synthesis itself has a many applications, ranging from developing more effective vaccinations to creating large synthetic gene libraries. Synbio Technologies offers the ability to take a requested genetic sequence, in text format, and create the desired physical sequence with one hundred percent accuracy. This is done with our, patent pending, Syno®2.0 Platform. The unique feature that this platform offers is the capability of synthesizing a genetic sequence that may not be preexisting within any organism. Previously, the sequence of interest was required to be existing in order to be extracted and later cloned. The Syno®2.0 Platform allows us to generate the user specified sequence exactly, confirmed with Sanger sequencing, with a both time and cost effective manner. This technology is the foundation for genetic engineering. Genetic engineering is defined as the alteration of an organism’s genome using biotechnology.

This is accomplished through the incorporation of a genetic sequence that is foreign to an organism into its genome. The unique connection between gene synthesis and genetic engineering is found within this step. Gene synthesis allows us to synthetically create the gene of interest, which is then incorporated into the organism’s genome. The gene of interest can have a wide range of functions, and are specific and tailor made to the interest of the researcher. Once the gene of interest is incorporated into the organism’s genome, research can be conducted to determine effects of the newly incorporated gene on the organism. This general outline has been applied to a wide range of applications in various fields of interest.

The applications of genetic engineering can be found in many fields of ranging from gene therapy, through the use of viral vectors, to agriculture. Gene synthesis and genetic engineering have been used to study the effects of a genetic knockout in a particular gene, a method that is very commonly used in genetic research. It is seen when studying the effects of gene amplification, causing an overexpression of a gene of interest. Genetic engineering is most commonly seen in agriculture through the creation of genetically modified crops to optimize the quality as well as quantity of certain crops. This process has been going on for thousands of years through selective breeding, but recent advances in technology have allowed researchers to implement groundbreaking innovations on basic crop production. These innovations help to combat environmental pressures, such as draught or blight, as well as decreasing pesticide use by genetic modification. These modifications, accomplished by genetic engineering, have a simple goal in mind: to increase the quality and quantity of these crops which are essential in everyday life. It is through genetic engineering and gene synthesis that these innovations are possible.

The main advantage that gene synthesis has when relating to genetic engineering is the accessibility and ease of synthetically creating your gene of interest. With Synbio Technology’s Syno®Platform the process from desired sequence to genetically modified organism is easily achieved. This process starts with the input of the researcher’s requested sequence, up to 100kb in length. The requested sequences is created with one hundred percent accuracy and verified with Sanger sequencing. This step is done with a both time and accuracy efficient pipeline. The desired sequence will then be shipped to your location within as few as five business days. In addition to the time and accuracy efficiency Synbio Technologies offers an extremely cost effective approach, with prices starting at $0.25 per base pair for gene synthesis. The combination of competitive pricing, time efficiency, and accuracy have lead Synbio Technologies to be one of the leading biotechnology companies, especially within the realm gene synthesis and its relationship with genetic engineering.

Effects of the Syno® Platform in Gene Synthesis Industry

Recently, gene synthesis has become an extremely popular and effective method to conduct genetic research. This rise in popularity stems from the ease of use as well as the effective results. In short, gene synthesis can be described as the creation of a synthetic genetic sequence specified by the user. Recently, the genetic sequence of interest must have been present within an organism to be extracted and later studied. Gene synthesis allows researchers to bypass this step entirely, creating the sequence of interest and converting the sequence from text format to physical copy with ease. This accessibility has caused multiple companies to participate in this groundbreaking technology within the field of genetics. As the gene synthesis industry has grown drastically, companies have competed to become the best at synthesizing genes. At Synbio Technologies we pride ourselves as being one of the leading companies within this gene synthesis industry. This pride and confidence is mainly relying upon our, patent pending, Syno® Platforms. It is the three phases of this platform that separates us from any other company in the gene synthesis industry, allowing us to serve you with a high quality product, with an efficient time frame and competitive prices.

The first platform that Synbio Technologies offers, the Syno®1.0 Platform, is an industrialized DNA chemical synthesis platform. Essentially, this platform specializes in the formation of oligos. An oligo can be described as a short fragment of DNA or RNA molecule. These oligos have a wide range of functions in various fields of genetic research including forensics, and genetic testing. In order to accomplish the output of high quality oligos, Synbio Technologies has four steps within this mechanism. First, the DNA sequence of interest is analyzed and designed. Once this complete, CG-base oligo synthesis is conducted. This step includes industry leading and manufacturing process which allows for the highest quality oligos. In order to increase the quality, the oligos are then purified using the proprietary purification process. Finally, there is then an additional step of quality assessment using MS analysis to verify the created oligos are what the user requested. The Syno® 1.0 Platform has a wide range of applications using this four part mechanism. Some applications include generating native DNA sequences, creating de novo DNA sequences, and constructing degenerated oligo libraries and so on. All of these processes have been utilized and proven with the use of oligo synthesis. This is just the first step in the process, which has allowed Synbio Technologies to become one of the premier companies in the gene synthesis industry. The remaining two platforms are what really separate us from the competition.

The Syno®2.0 Platform focuses on PCR based gene synthesis and is an aspect that Synbio Technologies prides itself as being one of the leaders in the biotechnology and gene synthesis industries. Synbio Technologies is capable of synthesizing a wide range of gene lengths up to and including 100kb. The process as to how these genes are synthesized are constantly verified for accuracy as well as quality. The resulting synthesized gene product is guaranteed to be one hundred percent identity to the user specified requested sequence. The resulting synthesized gene can then be applied in various types of genetic research. One major application that differs from many others is the ability to generate de novo DNA sequences. This aspect has revolutionized genetic research since the early 1970s, increasing in quality and effectiveness along the way. The creation of these de novo DNA sequences has allowed researchers to develop more effective vaccinations, generate variant libraries, and improve the features of protein. It is the basis of so many crucial fields of genetic research. At Synbio Technologies we offer the highest quality gene synthesis product with the most competitive prices in the gene synthesis industry. We pride ourselves with the accuracy of our product which we are able to provide our users.

The third and final platform, the Syno®3.0 Platform, specializes in oligo pool synthesis. This platform is described as a chip-based next generation DNA synthesis platform. Essentially, this platform allows us to generate the highest quality DNA fragments, de novo synthesized genes, small genomes, and variant libraries. In addition to this, the high quality output of these various genetic materials is provided to the user at the lowest prices in the gene synthesis industry. The process that this platform follows is very similar to that of the Syno®1.0 Platform with some small and necessary alterations. First the proprietary DNA sequences are designed, analyzed and optimized. Once this is complete, the chip-based oligo pool synthesis process begins. This step utilizes the, industry leading, oligo-pool assembly and manipulation process. After that, there is proprietary error correction, allowing for a quality control and improving the resulting output. Then large fragment assembly, up to 200kb, is conducted. Once this is finished, the final step is to run a quality control including next generation sequencing. This final step allows for the resulting sequence to be verified and one hundred percent accurate. Overall, the applications of this platform are similar to that of the previous Syno®1.0 and 2.0 Platforms. The user can generate native and de novo DNA sequences, build genes, chassis, operons, pathways and small genomes, as well as generate DNA variant libraries. In addition to these features, this platform offers a unique aspect which allows the process to be scalable. This means that it will take the same amount of time to produce 20,000 genes as it would to produce 20 genes, making the Syno®3.0 Platform more effective. These features are what make the Syno®3.0 Platform extremely valuable to many users.

Gene Synthesis Expression Vector

Gene synthesis is a fundamental technique in synthetic biology, allowing for the creation of unique synthetic genes that would be difficult or impossible to obtain naturally. In order to express a synthetic gene in an efficient and controlled way, expression vectors are often used to introduce the gene into a cell, after which the cell’s own gene expression system is used to synthesize the target protein.

Common Gene Synthesis Vector Types

There are three main categories of carriers that constitute vector systems in gene synthesis engineering: plasmids, bacteriophages and viruses. One of the most widely used expression vectors is that of Escherichia coli due to its ease of growth, rapid reproduction, and ease of transformation with exogenous DNA. Others include integration vectors, bacteriophage vector promotion, or shuttle vectors in S. cerevisiae.

Occasionally, a series of pGEX vectors (-1T, -2T, -3T), GST (glutathione S-trasferase) system, pEZZ18 are utilized for specific expression systems. Several variants transformed from pBluescript Ⅱ SK(+), together with pUC18, pUC19, pUC57, are also selected for certain projects.

Synbio Technologies are able to synthesize a large variety of target DNA and clone them into any vector as requested, regardless of the specifications of the project or the approach required. We guarantee a highly accurate and high-yield product at a low cost and with rapid turnaround.

Gene Synthesis Vector Design Application

Synbio Technologies provides two main approaches for amplifying DNA sequences via PCR cloning and subcloning technology. For DNA or amino acid sequences provided by customers, Synbio Technologies will synthesize error-free DNA de novo by request. Our proprietary Syno®gene synthesis platform can perfectly achieve enzymatic assembly through limited short DNA fragments. Even genes with challenging characteristics such as repetitive sequences, complex secondary structure, and high (>80%) or low (20%) GC content as well as long polypyrimidine runs, can be synthesized correctly.

Additionally, our professional Syno®Codon software can compute and optimize codon selection for original sequences or specific vectors. Any synthetic vector meeting our requirements can be quickly and accurately delivered at an economical price. Synbio Technologies can insert any target gene into any site of any vector, and can guarantee a high-quality, fully accurate final product for gene synthesis.